Measurement instruments
Collected information in MOTAR
| Instrument | Method | Week 0 |
Week 6 | Week 10 | Week 16 | Week 52 | |
| Primary outcomes | |||||||
| Biological aging: telomere length, telomerase activity | Fasting blood samples | Blood | X | – | – | X | X |
| Secondary outcomes | |||||||
| Biological and general health indicators | |||||||
| Biomarkers (inflammation, metabolic syndrome) | Fasting blood samples | Blood | X | – | – | X | X |
| Gene-expression (RNA) | Fasting blood samples | Blood | X | – | – | X | X |
| HPA-axis (cortisol) | 2 days of 6 saliva samples | Saliva | X | – | – | X | X |
| Oxidative stress | Urine sample | Urine | X | – | – | X | X |
| Autonomic nervous system | Electro + impedance cardiography, heart rate variability | ME | X | – | – | X | X |
| Blood pressure | Systolic and diastolic BP | ME | X | – | – | X | X |
| Body composition | Weight, height, waist+ hip circumfereence | ME | X | – | – | X | X |
| Physical condition | Astrand sub max test | ME | X | – | X | X | |
| Muscle strength | Hand grip strength | ME | X | – | – | X | X |
| Lung function | Peak expiratory flow | ME | X | – | – | X | X |
| Pain | Chronic graded pain scale | Int | X | – | – | X | X |
| Somatization | Short somatization questionnaire | SR | X | – | – | X | X |
| Disability severity | WHO-DAS II | SR | X | – | – | X | X |
| Depressive and anxiety disorders | |||||||
| Presence of MDD | CIDI: MDD | Int | X | – | – | X | X |
| Presence of anxiety dis | CIDI: SocPhob, Agora, GAD, PA | Int | X | – | – | X | X |
| Course of symptoms | Life-chart | Int | X | – | – | X | X |
| Severity of depression | Inventory of depressive symptoms | SR | X | X | X | X | X |
| Severity of anxiety | Beck anxiety index and Fear questionnaire | SR | X | X | X | X | X |
| Sleep | Insomnia Rating Scale | Int | X | – | – | X | X |
| Descriptive variables, potential confounding covariates and potential mediating variables | |||||||
| Age, gender, ethnicity | Standard questions | Int | X | – | – | – | – |
| Socio-economic status | Education, income, occupation | Int | X | – | – | – | – |
| Physical activity | SQUASH questionnaire | SR | X | – | – | X | X |
| Smoking | Past + current smoking questions | SR | X | – | – | – | X |
| Medication use | Drug container observation | Int | X | – | – | X | X |
| Regular alcohol intake | AUDIT questionnaire | SR | X | – | – | – | X |
| Somatic diseases | Presence + symptoms of disease | Int | X | – | – | X | X |
| Health care | Perceived need of care | Int | X | – | – | X | X |
| Work and disability | Tic-P questionnaire | Int | X | – | – | X | X |
| Personality | NEO-FFI questionnaire | SR | X | – | – | X | X |
| Locus of control | Pearlin & Schooler mastery scale [103] | SR | X | – | – | X | X |
| Depression vulnerability | LEIDS-R questionnaire | SR | X | – | – | X | X |
| Anxiety vulnerability | Anxiety senstivity index | SR | X | – | – | X | X |
| Experimental cognitive task | Implicit association test (IAT) | CT | X | – | X | X | |
| Experimental memory task | Digit Span (WAIS) | Int | X | – | – | X | X |
| Important neg + pos life events | Brugha questionnaire | Int | X | – | – | X | X |
| Childhood Trauma | Youth Trauma questionnaire | SR | X | – | – | – | – |
| Familiy history | Familiy history inventory | Int | X | – | – | – | – |
| Neuroimaging assessment (subsample) | |||||||
| Verbal episodic memory | 15-words test | MRI | x | – | – | x | x |
| Task-related brain activity | Emotional face matching paradigm and N-back paradigm | MRI | x | – | – | x | x |
| Brain network connectivity | Resting state MRI images | MRI | x | – | – | x | x |
| Process indicators (intervention adherence) | |||||||
| Exercise intervention group: Exercise participation and heart rate will be administrated during each session.. | |||||||
| Antidepressant intervention group | Side effect medication questionnaire | Int | – | X | X | X | – |
| Adherence (pill count) | Int | – | X | X | X | – | |
SR=self-report, Int=interview, Blood=data collection via fasting blood sample, CT=computer task, ME=medical examination
